Summary
Type: SPE
Application #: 305510
Catalog#: 730626 - SPE Cartridges, Chromabond, NH2, 6ml, PP, 1000mg 30/pk
Detection:
Conditions: Column conditioning: no information from author; recommended 10 mL chloroform
Sample aspiration: 50 mg of each total lipid mixture solved in chloroform
The individual lipid classes are eluted with solvent mixtures of incresing polarity:
Elution 1: neutral lipids with 12 mL chloroform – isopropanol (2:1)
Elution 2: free fatty acids with 10 mL diethyl ether – acetic acid (98:2)
Elution 3: phospholipids with 10 mL methanol
Evaporation: phospholipid fraction is evaporate to dryness
Reconstitution: chloroform; for HPLC separation phospholipid solution of 0.5 mg/mL in methanol – chloroform (2:1) is prepared
Authors: Schwalbe-Herrmann, M. et al.
Source: J. Chromatogr. A 1217 (2010), 5179–5183